Clinical Chemistry

A Comprehensive Assessment of Metagenomic cfDNA Sequencing for Microbe Detection

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Clin Chem. 2025 Apr 24:hvaf044. doi: 10.1093/clinchem/hvaf044. Online ahead of print. ABSTRACT BACKGROUND: Metagenomic cell-free DNA (cfDNA) sequencing provides a new avenue for diagnosing infectious diseases. Owing to the low concentration and highly fragmented nature of microbial cfDNA in plasma, coupled with methodological complexity, ensuring accurate and comparable metagenomic cfDNA sequencing results has proved challenging. … Read more

MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines

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Clin Chem. 2025 Apr 24:hvaf043. doi: 10.1093/clinchem/hvaf043. Online ahead of print. ABSTRACT BACKGROUND: In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines established standards for the design, execution, and reporting of quantitative PCR (qPCR) in research. The expansion of qPCR into numerous new domains has driven the development of new … Read more

A Comprehensive Review of Estradiol, Progesterone, Luteinizing Hormone, and Follicle-Stimulating Hormone in the Context of Laboratory Medicine to Support Women’s Health

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Clin Chem. 2025 Apr 18:hvaf039. doi: 10.1093/clinchem/hvaf039. Online ahead of print. ABSTRACT BACKGROUND: There have been conflicting messages about the influence of female sex hormones on women’s health, with historical messaging indicating that use of estrogen and/or progesterone in peri- or postmenopause poses a significant clinical risk to cisgender women. More recent guidance indicates that … Read more

Instrument Multiplexing: Amplified Throughput for Measurement of Vitamin B1 in Whole Blood Using Parallel Liquid Chromatography Systems Interfaced to a Single Mass Spectrometer

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Clin Chem. 2025 Apr 9:hvaf029. doi: 10.1093/clinchem/hvaf029. Online ahead of print. ABSTRACT BACKGROUND: Thiamine diphosphate (TDP), the active form of vitamin B1, plays an essential role in energy metabolism. TDP is analyzed in the clinical laboratory to assess the nutritional status of individuals at risk of deficiency. In recent years, demand for vitamin B1 testing … Read more

Methylglyoxal-Induced Glycation of Plasma Albumin: From Biomarker Discovery to Clinical Use for Prediction of New-Onset Diabetes in Individuals with Prediabetes

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Clin Chem. 2025 Apr 7:hvaf035. doi: 10.1093/clinchem/hvaf035. Online ahead of print. ABSTRACT BACKGROUND: Methylglyoxal (MGO) is a potent glycating agent that contributes to the pathogenesis of diabetes. However, MGO is unstable in plasma without demanding sample preparation at blood collection, limiting its clinical utility as a biomarker. We aimed to discover reliable MGO-glycated albumin (ALB)-derived … Read more

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