A Digital PCR Assay for Fetal Fraction Quantification Using Multiplex SNP and Y Chromosome Detection
Clin Chem. 2026 Feb 26:hvag017. doi: 10.1093/clinchem/hvag017. Online ahead of print. ABSTRACT BACKGROUND: Fetal fraction (FF) quantification is critical for prenatal cell-free DNA screening. Current methods based on Y chromosome (chrY), differential methylation, or single-nucleotide polymorphisms (SNPs) face limitations including sex dependency, complex workflows, or the need for parental genotyping. An FF quantification method that … Read more