Allele-Specific Digital PCR Enhances Precision and Sensitivity in the Detection and Quantification of Copy Number Alterations in Heterogeneous DNA Samples

Clin Chem. 2025 Jun 19:hvaf054. doi: 10.1093/clinchem/hvaf054. Online ahead of print. ABSTRACT BACKGROUND: While genetic analyses are vital in cancer diagnostics, measuring copy number (CN) alterations remains challenging, especially in heterogenous samples such as (liquid) biopsies. Using digital PCR, CN alterations are classically studied by comparing the abundances of the target of interest to a … Read more

eGFR Inaccuracy in the Assessment of Living Kidney Transplant Donor Eligibility

Clin Chem. 2025 Jun 18:hvaf071. doi: 10.1093/clinchem/hvaf071. Online ahead of print. ABSTRACT BACKGROUND: Accurate glomerular filtration rate (GFR) estimation is crucial for evaluating living kidney donors, especially when measured GFR (mGFR) is unavailable. This study compares the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI), full age spectrum (FAS), and European Kidney Function Consortium (EKFC) creatinine- and/or … Read more

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